Synthesis of nZVI/PVP nanoparticles for bioremediation applications

The objective of this investigation is to synthesize and investigate zero-valent iron (ZVI) nanoparticles (NPs) for bioremediation applications. ZVI-NPs were fabricated by chemical reduction using a ferrous salt solution with poly(N-vinylpyrrolidone) (PVP), used as stabilizer. synthesis was conducted without ultrasonic treatment. ZVI NPs characterized scanning electron microscopy (SEM), transmission (TEM), X-ray powder diffraction (XRD) analysis, Fourier Transform Infrared Spectroscopy (FTIR). Experimental observations demonstrate that depending on conditions coordination stabilizers, different morphologies are formed. Colloidal solutions the synthesized in antimicrobial activity tests biofilm formation assays nine control microorganisms: Escherichia coli (ATCC 25922), Pseudomonas aeruginosa 15692), Enterococcus faecalis 29122), Klebsiella pneumoniae (laboratory isolates), Proteus vulgaris Staphylococcus aureus 29213), Bacillus cereus (DSMZ 4312), subtilis 6633), Candida albicans 10231). All strains did not show antibacterial effect against PVP-stabilized However, results highest absorbance values micro-organisms tested wells. Although B. subtilis, E. coli, K. observed during formation, cereus, S. aureus, P. reduced noticeably Fe0/PVP-US (A1) NPs. For strains, such C. albicans, no observed. Fe0/PVP (A2) NPs, faecalis, pneumoniae, vulgaris, demonstrated positive effect, showed negative effect. A strategic utilization nZVI-PVP great potential effective, efficient, sustainable